benzonase
Appearance
English
[edit]Etymology
[edit]From benzon (nucle)ase.
Noun
[edit]benzonase (countable and uncountable, plural benzonases)
- (genetics) An artificial endonuclease used to degrade DNA and RNA.
- 1991 October, J. M. Moreno, “Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase”, in Applied Biochemistry and Biotechnology volume, volume 31:
- Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob.
- 1999, Andrew J. Link, 2-d Proteome Analysis Protocols, page 83:
- The volume of benzonase solution (ready-made solution of the manufacturer) necessary to digest the DNA in the pellet III suspension was determined experimentally.
- 2000 October, P. Ravindra, Anupama, Value-added food:: Single cell protein, in Biotechnology Advances, volume 18, issue 6, pages 459-479:
- Immobilized nucleases like benzonases on corncobs were also used to reduce the nucleic acid content in protein concentrates.
- 2011, Charles Y. Lee, Korkut Uygun, Methods in Bioengineering: Organ Preservation and Reengineering, page 185:
- Treat the lung with benzonase to remove the remnant DNA:
- 2014 May 23, Muniasamy Neerathilingam, “Soni-removal of nucleic acids from inclusion bodies”, in Biochemical and Biophysical Research Communications, volume 448, number 1, page 45:
- Also, in many cases, enzymes like benzonases fail to completely remove nucleic acids from complexes due to inaccessibility of shorter nucleic acids buried in the refolded protein as demonstrated in this study.
- 2015, Antonio Monari, Elise Dumont, Chryssostomos Chatgilialoglu, Radiation-induced and oxidative DNA damages, page 42:
- The new digestion protocol was designed based on the enzymatic combination of benzonase and P1 nucleases.
Usage notes
[edit]Capitalized when referring to a commercial product.